Why am I cloning crocodiles into antibiotic resistant bacteria cells?
- Oct 9, 2015
- 2 min read
I’m a sailor, molecular biologist and scuba diver, and oh yeah, I just cloned stuff and inserted antibiotic resistance and crocodile genes into E.Coli cells. Don’t worry; I know what I’m doing – I’m not creating Godzilla. Yet. I’m back in the lab, where I like Dexter in Dexter’s laboratory perform bizarre experiments that apparently seem crazy and difficult. It is not until I speak with people who haven’t studies natural sciences that I realize that the five years at the University hasn’t been wasted – I have indeed learnt something. When I causally can talk about copying DNA, cloning cells and looking for things too small for the eye to see, and the person I am talking to looks at me as if I was an alien, then I know, that I have learnt something. Recently I have received a lot of questions about what it is I am doing in the lab 8 hours a day – what am I looking for? So I have come up for this analogy that most people seem to understand. So DNA represents the blueprints for our body, and each cell in your body has a master blueprint. Now imagine you want to build a house. You wouldn’t want to bring the master blue print out to the construction site, I mean, someone might spill coffee on it, drop it into the mud, and then it could get destroyed, it’s dangerous because then you can’t build any more houses. So what would you do? You’d make a copy and bring it to the construction site. This copy is called mRNA. Now imagine that you are building several houses. Naturally, you would make several copies, to bring to each construction site. Now, I am not looking for a house. I am looking for enzyme that is needed if an animal lives in a really contaminated area. Instead of looking for the contaminants themselves, I am looking for the thing that cleans it up: the broom. And the logic follows that the more brooms, the dirtier the house. Makes sense right? But where does the cloning come in? Why am I making E. Coli resistant to antibiotics? Well, I’ve glued the blueprint for the broom onto a circle of blueprints that can enter the cells, and that makes it resistant to antibiotics. And then I try to grow the cells in their favorite environment, with the exception to antibiotics being present. Only the bacteria that have started producing my “broom” and antibiotic resistance will survive. And that’s how I can find my “broom”, and later sequence it so I can see exactly what it’s made up out of.


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